Fig 1: Microphotos of primary cell culture of the mouse mesencephalon (A–D) and metencephalon (E–L) (7th day of cultivation) after the following procedures: (i) phase contrast photography (A,E,I); (ii) incubation with 50 nM GBR-BP and photography of GBR-BP-stained fluorescent cells (B,F,J); (iii) merging the images obtained in “i” and “ii” (C,G,K); (iv) fixation with 4% paraformaldehyde with loss of GBR-BP fluorescence followed by fluorescent immunostaining of mesencephalic cells for tyrosine hydroxylase ((TH); dilution 1:500) (Abcam, ab19353) (D) and metencephalic cells for dopamine-ß-hydroxylase ((DBH); dilution 1:500) (Chemicon, ab1542)) (H) or 5-hydroxytryptamine (5-HT; dilution 1:750) (Sigma, S5575) (L) and DAPi staining of neuronal nuclei. Objective × 20 (Plan-Neufluar). Bar = 50 µm.